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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: Neuroscience. 2020 Jun 13;441:33–45. doi: 10.1016/j.neuroscience.2020.06.009

Figure 5. Elevated HA signaling by VPS35 D620N.

Figure 5.

Differentiated SH-SY5Y cells expressing wild-type (WT) or D620N VPS35 were serum-deprived overnight. (A) The following day, high molecular weight (>950 kDa) HA (300 μg/mL) or (C) low molecular weight (15 – 40 kDa) HA (300 μg/mL) was added in serum-free media for the indicated times and western blotting was then performed (p-AKT = Ser473). (B, D) Results are presented as a ratio of D620N/WT for p-AKT/β-Actin by western blot band densitometry for each time point shown (*p<0.05 versus indicated group, student’s t-test; Error bars = SEM).