Figure 2.

High Transduction Efficiency of Anc80L65 in Cochlear HCs

(A) The organ of Corti was mounted and divided into three parts: the apex, middle, and base. One row of IHCs and three rows of OHCs are clearly visible in the images. We compared myosin VIIA and eGFP expression between the Anc80L65-injected ears and the sham ears (injection of PBS solution only). Red indicates myosin VIIA, green indicates eGFP, and blue indicates DAPI. The scale bar represents 50 μm (n = 5). (B) The transduction efficiency was quantified in cochlear HCs with eGFP expression. No significant difference between the IHC and OHC transduction efficiency was found in any turn. (C) Supporting cell layer in a whole-mount preparation of the middle cochlear turn (same as in A). Sham ears were utilized as a positive control for Sox2 and a negative control for eGFP. Gray indicates Sox2-positive supporting cells, and green indicates eGFP. The scale bar represents 50 μm.