Figure 4.

MIMS Signature of Metabolic Heterogeneity Is Not Generalizable to Non-malignant Cells

(A) Representative images of small intestine. Top: villus lined by terminally differentiated, non-dividing (BrdU⁻) epithelial cells and demonstrating a uniform stripe of labeling corresponding to the brush border (arrows). Bottom: Paneth cells identifiable by their stereotypical sulfur-rich granules (arrows) are surrounded by proliferating cells (BrdU⁺) that are stem/transit amplifying cells. Scale bar, 10 μm.

(B) Dot plot of cells from (E). Individual cell types (color coded) segregate by labeling intensity. Note: only the stem/TA cells are proliferative (BrdU⁺). TA, transit amplifying cell.

(C) Mitotic figure from MPNST. Segregating chromosomes (arrows), evident in the ³¹P images, are also BrdU labeled. Scale bar, 5 μm.

(D) Dot plot demonstrating heterogeneity of glutamine and glucose labeling of MPNST mitotic figures relative to mitotic figures from the small intestinal crypt as also indicated by the median absolute deviation: MPNST glucose (MAD = 54.8) and glutamine (MAD = 35.5) versus crypt glucose (MAD = 36.3) and glutamine (MAD = 1.6).