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. 2020 Jun 2;24:103–111. doi: 10.1016/j.jot.2020.05.004

Fig. 7.

Fig. 7

IHC results of key proteins in Ihh signaling at the tendon-bone interface in Group 1–5 mice. (A–E) Expression of Ihh, Ptch1, Smo, Gli2, Gli3 proteins at the tendon-bone interface. (F) IHC semi-quantitative expression results of key proteins in Ihh signaling using H-score at the tendon-bone interface in Group 1–5 mice. The H-score ranged from 0 to 300 was used for semi-quantitative assessment of IHC images according to both the intensity of staining and the percentage of cells stained. The intensity was considered 0 for absent expression, 1+ for weak staining, 2+ for moderate staining, and 3+ for strong staining. The H-score was calculated as the formula: H-score = 1 × percentage of 1+ cells +2 × percentage of 2+ cells +3 × percentage of 3+ cells. Scale bars = 50 μm. Data are expressed as the Mean ± S.D. ∗p < 0.05; ∗∗p < 0.01; n.s. indicates no significant difference.