Figure 2.

circREPS2 Suppressed the Proliferation, Colony Formation, and DNA Synthesis Abilities of GC Cells

(A and B) Quantitative real-time PCR experiment confirmed that circREPS2 was successfully overexpressed in BGC-823 and SGC-7901 cell lines (A) and knocked down in MKN-45 cell line (B). (C) The expression of linear host gene REPS2 in in BGC-823, SGC-7901, and MKN-45 cells accessed by quantitative real-time PCR. (D and E) CCK-8 (D) and colony-formation assay (E) analysis of cell proliferation viability after transfection of the circREPS2 overexpression vector in BGC-823 and SGC-7901 and si-circREPS2 in MKN-45 cells. (F–I) EdU assay detected positive stained cell percent when overexpressing or knocking down circREPS2 in BGC-823 (F and I), SGC-7901 (G and I), and MKN-45 (H and I), respectively (scale bar, 50 μm). Values are shown as the mean ± standard error of the mean based on three independent experiments. ∗p < 0.05, ∗∗p < 0.01.