Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 28;16:100. doi: 10.1186/s13007-020-00642-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Recognition and qualification of Populus trichocarpa embryo cells by ImageJ and Imaris and their comparison. a Raw image of a Populus trichocarpa embryo captured by light sheet fluorescence microscopy (LSFM). b Compensation for the non-homogeneous fluorescent signal distribution in a using the ‘Plane Brightness Adjustment’ plugin. c Image of cell recognition and qualification by ImageJ software. d Quantification of cell area, perimeter, and Feret’s diameter from c. Boxplots represent mean, 25th, and 75th quartiles, whiskers represent minimum and maximum. n = 5845. e Image of cell recognition and qualification by Imaris software. f Heatmap of cell area calculated from e. The color scale represents the cell areas. g–j Comparison of values calculated by ImageJ and Imaris software. Statistical diagram of total cell number (g), total cell area (h), average cell area (i), and relative frequency of cell area. Boxplots represent mean, 25th, and 75th quartiles, whiskers represent minimum and maximum. n = 5845 and 6070. k Heatmap of correlation matrix analysis (Pearson, confidence interval = 95%) between cell areas (each cell area dataset is divided into 25 groups) calculated by ImageJ and Imaris software. In a–c, e, and f, panels on the right show enlarged images of areas on the left highlighted with white boxes; bar = 200 and 50 μm respectively