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. 2020 Jul 28;16:100. doi: 10.1186/s13007-020-00642-0

Fig. 5.

Fig. 5

Recognition and qualification of cells in an Arabidopsis seed section. a Images of Arabidopsis seed cells captured by micro-computed tomography (Micro-CT) and images after clearness, threshold adjustment, and particle recognition, bar = 50 μm. b Graphical user interface of ImageJ software, including the main interface, ROI manager panel, and results display window. An ROI list and cellular parameters characterized from Arabidopsis seed cells in a. cf Cellular parameters of Arabidopsis seed cells. Quantification of area, perimeter, and Feret’s diameter of Arabidopsis seed cells in a. Boxplots represent mean, 25th, and 75th quartiles, whiskers represent minimum and maximum, n = 593 cells (c). Relative frequency distribution (percentage, %) analysis of area (d), perimeter (e), and Feret’s diameter (f) shown in c