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. 2020 Jul 28;16:100. doi: 10.1186/s13007-020-00642-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Recognition and qualification of cells in a transverse section of a Populus trichocarpa stem. a Images of Populus trichocarpa stem cells captured by light microscopy (LM) after staining with toluidine blue and images after clearness, threshold adjustment and particle recognition, bar = 100 μm. b Graphical user interface of ImageJ software, including the main interface, ROI manager panel, and results display window. ROI list and the cellular parameters characterized from Populus trichocarpa stem cells in a. c–f Cellular parameters of Arabidopsis seed cells. Quantification of area, perimeter, and Feret’s diameter of Arabidopsis seed cells in a. Boxplots represent mean, 25th, and 75th quartiles, whiskers represent minimum and maximum, n = 1243 cells (c). Relative frequency distribution (percentage, %) analysis of area (d), perimeter (e), and Feret’s diameter (f) shown in c