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. 2020 Jul 28;16:100. doi: 10.1186/s13007-020-00642-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Analysis of Populus trichocarpa stem cell organization by tessellation-based automatic segmentation of an LM image. a Illustration of a centroid coordinate read-out in Populus trichocarpa stem cells produced by ImageJ. Green arrows indicate the direction of the x and y axes, and the number indicates the centroid coordinate of the red point. b Graphical user interface of SR-Tesseler software, including the console, control window, and viewer panel. c Merged image of Populus trichocarpa stem cell particles identified by ImageJ and centroid (red points) displayed in the SR-Tesseler viewer window. d–f Segmentation and quantification of experimental data according to the centroid in c. Polygon creation and establishment of Voronoï diagrams based on the local density (d), mean distance (e), and area (f). Empty polygons are shown above, and filled polygons are shown below. All polygons were merged with the particles identified from Populus trichocarpa stem cells. The polygons were pseudocolor-coded with respect to the segmentation results. g The connection pattern of clusters calculated from established objects. All bars in this figure represent 10 μm