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. 2020 Jul 23;14:45. doi: 10.3389/fncir.2020.00045

Figure 2.

Figure 2

Identification of CS-Parv neurons in the auditory cortex of wild-type C57BL/6 mice. (A) Schematic depicting the injection site of red RetroBeads using the wild-type C57BL/6 mouse line to identify CS-Parv neurons in the auditory cortex. Bottom, striatum: red RetroBeads injection site; Top, auditory cortex: red beads retrolabeled pyramidal and CS-Parv neurons identified by immunostaining with anti-Parv. (B) Left: overlay image of red-positive retrograde labeled neurons in the auditory cortex identified by injection of retrograde beads in the dorsal striatum and anti-Parv in the wild-type C57BL/6 mouse line. Top, right: anti-Parv immunostained Parv neurons in the auditory cortex in the wild-type C57BL/6 mouse line. Middle, right: CS-Parv neurons identified by anatomical retrograde labeling in the wild-type C57BL/6 mouse line. The arrow indicates the location of the red beads in the CS-Parv neurons. Bottom, right: overlay of CS-Parv neurons immunostained with anti-Parv and retrograde beads labeled CS-Parv neurons. The arrow indicates the location of the red beads in the CS-Parv neurons.