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. 2020 Jul 23;13:118. doi: 10.3389/fnmol.2020.00118

Table 1.

Sites of laminin-α2 expression in the developing and adult central nervous system.

Location Embryonic Postnatal* Adult**
Astrocytes at the blood-brain barrier Not available Immunofluorescence
Human:
- α2 chain mouse mAb, 80 kDa specific; α2 chain rat mAb, 300 kDa specific (Villanova et al., 1997).
Mouse:
- α2 chain rat mAb, N-terminus specific (Menezes et al., 2014)
Protein, western blotting
Mouse:
- α2 chain rat mAb, N-terminus specific (Menezes et al., 2014)
Immunofluorescence
Human:
- α2 mouse mAb, 80 kDa specific, and α2 rat mAb, 300 kDa specific (Villanova et al., 1997).
Mouse:
- α2 chain rat mAb, N-terminus specific (Menezes et al., 2014)
- α2 chain mouse mAb, 300 kDa specific (Hannocks et al., 2018)
- α2 chain mouse mAb, 300 kDa specific (Sixt et al., 2001)
- α2 chain rat mAb, N-terminus specific (Yao et al., 2014)
Cortical plate Immunofluorescence
Mouse:
- α2 chain (Campos et al., 2004)
- α2 chain rat, N-terminus specific (Lathia et al., 2007)
Not available Not available
Choroid plexus RNA, northern hybridization
Human:
- α2 chain, multiple nucleotide residues screened using previously reported cDNA (Vuolteenaho et al., 1994)
Not available Not available
Cerebellum RNA, northern hybridization
Human:
- α2 chain, multiple nucleotide residues screened using previously reported cDNA (Vuolteenaho et al., 1994)
Immunofluorescence
Mouse:
- α2 chain mouse mAb, N-terminus (Powell et al., 1998) Protein, western blotting
Mouse:
- α2 chain mouse mAb, N-terminus (Powell et al., 1998)
Not available
Hippocampus Protein, western blotting
Rat:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997)
Immunofluorescence
Rat:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997)
Immunofluorescence
Monkey:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997) Rabbit:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997) Rat:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997) - α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997) Protein, western blotting
Mouse:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997) Pig:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997) Rabbit:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997)
Rat:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997)
Meninges RNA, northern hybridization
Human:
- α2 chain, multiple nucleotide residues screened using previously reported cDNA (Vuolteenaho et al., 1994)
Not available Immunofluorescence
Mouse:
- α2 chain rabbit polyclonal Ab, C-terminus domain G1–3 specific (Sasaki et al., 2002)
Immunofluorescence
Mouse:
- α2 chain rabbit polyclonal Ab, C-terminus domain G1–3 specific (Sasaki et al., 2002)
Cortex (i.e., entorhinal and piriform cortices) Not available Not available Immunofluorescence
Rabbit:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997) Rat:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997) - α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997) Protein, western blotting
Pig:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Tian et al., 1997)
Olfactory bulb RNA, northern hybridization
Human:
- α2 chain, multiple nucleotide residues screened using previously reported cDNA (Vuolteenaho et al., 1994)
Not available Immunofluorescence
Rabbit:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997)
Pericytes Not available Immunofluorescence
Mouse:
- α2 chain rat mAb, N-terminus specific (Menezes et al., 2014) - α2 chain rat mAb, N-terminus specific (De La Fuente et al., 2017)
Protein, western blotting
Rat - α2 chain rat mAb, N-terminus specific (De La Fuente et al., 2017)
Immunofluorescence
Mice:
- α2 chain rat mAb, N-terminus specific (De La Fuente et al., 2017)
RNA, in situ hybridization
Rat - α2 chain rat mAb, N-terminus specific (De La Fuente et al., 2017)
Retina RNA, northern hybridization
Human:
- α2 chain, multiple nucleotide residues screened using previously reported cDNA (Vuolteenaho et al., 1994)
Not available Not available
Spinal cord Immunofluorescence
Mouse:
- α2 chain rabbit polyclonal Ab, C-terminus domain G1–3 specific (Sasaki et al., 2002)
Human:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific; α2 chain mouse mAb, 80 kDa fragment specific (Liesi et al., 2001)
Not available Not available
Thalamus and hypothalamus Not available Not available Immunofluorescence
Rabbit:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997)
Rat:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997)
Tanycytes Not available Not available Immunofluorescence
Rabbit:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997)
Rat:
- α2 chain rabbit polyclonal Ab, C-terminus domain G4/5 specific (Hagg et al., 1997)
Ventricular-subventricular zone Immunofluorescence
Mouse:
- α2 chain (Campos et al., 2004)
Immunofluorescence
Mouse:
- α2 chain (Campos et al., 2004)
Not available
- α2 chain rat mAb, N-terminus specific (Lathia et al., 2007)
Rat:
- α2 chain (Campos et al., 2004)
- α2 chain rat mAb, N-terminus specific (Relucio et al., 2012)
Rat:
- α2 chain (Campos et al., 2004)

Note: *postnatal day 0–20. **postnatal day 21 or greater.