Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 23;10:330. doi: 10.3389/fcimb.2020.00330

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Wang, Zhang, Lv, Deng and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Myricetin inhibits the hemolytic activity of Hla. (A) Chemical structure of myricetin. (B,C) Inhibition of Hla activity by myricetin (Myr). The hemolytic activity of purified Hla (5 μg/ml) pretreated with the indicated concentrations of myricetin was determined using a hemolysis assay. (D,E) Hemolytic activity of the supernatants from S. aureus co-cultured with myricetin. The sample treated with DMSO was regarded as a negative control, and the sample treated with Hla or the supernatants from 8325-4 strain in the presence of DMSO served as a positive control (100% hemolysis). (F) Growth curves of S. aureus in the presence of various concentrations of myricetin. (G) Western blot assay for Hla expression in the supernatants and precipitates of S. aureus cultures treated with or without myricetin. The gray value of Hla expression in the culture supernatants (H) and precipitate (I) of S. aureus cultures treated with or without myricetin using Image-Pro Plus 6.0 software. Each column shows the mean ± SD of three independent experiments and analyzed by the one-way ANOVA. *P < 0.05 and **P < 0.01 compared to the positive group.