TABLE 2.

Genetic determinants affecting susceptibility to all antimicrobial peptides tested (PMB, COL, L27-11, peptides 3 and 4).

Locus tag	Orthologs (PAO1)	Gene symbol	Description	Location (COG)a	PMBb	COLb	L27-11b	4b	3b
PA14_63150	PA4776	pmrA	Two-component response regulator	Cytoplasm (T)	−5.87	−4.86	−3.65	N/A	−4.00
PA14_63160	PA4777	pmrB	PmrB: two-component regulator system signal sensor kinase PmrB	CM (T)	−6.21	−7.19	−6.36	−6.25	−5.55
PA14_49180	PA1179	phoP	Two-component response regulator PhoP	Cytoplasm (T)	−2.80	−1.26	−4.05	N/A	N/A
PA14_18330	PA3556	arnT	4-amino-4-deoxy-L-arabinose transferase	CM (M)	−5.04	−5.03	−4.62	−4.56	−5.09
PA14_18340	PA3555	arnD	Hypothetical protein	Cytoplasm (G)	−3.93	−4.92	−4.62	N/A	N/A
PA14_18350	PA3554	arnA	Bifunctional UDP-glucuronic acid decarboxylase/UDP-4- amino-4-deoxy-L-arabinose formyltransferase	Cytoplasm (G,J,M)	N/A	N/A	−4.30	N/A	−7.58
PA14_18360	PA3553	arnC	Glycosyl transferase family protein	CM (M)	−4.99	−4.97	−3.96	N/A	N/A
PA14_18370	PA3552	arnB	UDP-4-amino-4-deoxy-L-arabinose–oxoglutarate aminotransferase	Cytoplasm (E)	N/A	−5.10	−3.80	−5.25	−5.78
PA14_58090	PA4476	asmA	Hypothetical protein	OM (S)	−4.56	−1.58	−1.16	−1.67	−1.68
PA14_42920	PA1667	tssK-2	HsiJ2	Cytoplasm (S)	−3.34	−3.47	−3.02	−3.07	−3.06
PA14_27190	–	–	tRNA-Ser	–	−1.87	N/A	−2.10	−1.94	−1.47
PA14_48970	PA0720	–	Helix destabilizing protein of bacteriophage Pf1	–	−4.04	−3.03	−2.42	−4.19	−1.91
PA14_49000	PA0717	–	Hypothetical protein	–	−1.48	−1.80	−1.67	−1.54	−1.28

^aCOG, clusters of orthologous group; T, signal transduction; M, cell wall/membrane/envelope biogenesis; G, carbohydrate metabolism and transport; J, translation; E, amino acid metabolism and transport; S, function unknown; CM, cytoplasmic membrane; OM, outer membrane. ^bLog2-fold change of normalized unique insertion densities (nUIDs) between the treated or untreated samples. N/A, not applicable as the fold change (FC) could not be calculated due to zero UIDs in the treated sample.