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. 2020 Jul 23;11:1755. doi: 10.3389/fmicb.2020.01755

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Copyright © 2020 Fiallo-Olivé and Navas-Castillo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Restriction fragment length polymorphism (RFLP) analysis of begomoviruses and deltasatellites infecting Corchorus siliquosus (samples 705 and 706). (A) RFLP performed by digestion of rolling circle amplification (RCA) products obtained from DNA extracts with the restriction enzyme HpaII revealed on a 1.5% agarose gel. (B) In silico RFLP analysis (HpaII) of the begomovirus and deltasatellite sequences obtained from each sample, performed with SnapGene (Insightful Science, available at snapgene.com). MW: molecular weight marker (HyperLadder 1 kb, Bioline).