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. 2020 Jul 23;8:819. doi: 10.3389/fbioe.2020.00819

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Copyright © 2020 Caramazza, Nardoni, De Angelis, Paolicelli, Liberti, Apollonio and Petralito.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Profile in time of the experimental release of the fluorescent dye. (A) Percentage release of 5–(6) CF monitored in time up to 2.5 h for four different trials: two exposed samples using 2000 pulses and 2 Hz (dark and light blue) and two temperature control samples (dark and light green). (B) Spontaneous release of 5-(6) CF from liposomes due to the operative temperature of 37°C up to 2.5 h via spectrofluorimetry. A set of four different trails is reported (yellow, blue, red, magenta) with the calculated mean curve (green) and the standard deviation values as bars.