Figure 3.

Visible light mapping and X-ray imaging process at the TXM

(A and B) Brightfield (A) and fluorescence (B) in line visible-light imaging of a sample grid supporting a mixed fluorescence cell population.

(C) 2D X-ray mosaic in the ROI denoted in (B) (green outline with individual FOVs delineated by hashed lines).

(D) Middle slice from the X-ray tomogram collected in the ROI denoted in (C) (orange outline). Characteristic cellular features are identified by arrows: red for nuclear envelope, purple for mitochondria, green for MVBs, blue for the contact area between two adjacent cells, and yellow for reovirus-carbon-rich structures within vesicles.