Figure S6.

The 3D organization of virus-containing structures within cytoplasmic vesicles, related to Figure 6

(A and B) In (A), is a single Z axis slice of an ROI X-ray tomogram showing two infected cells at 2 h after infection (respective nuclei at top and bottom of left-hand side) and in (B) is the same with all pixel values inverted (necessary for volume rendering in Chimera) (Pettersen et al., 2004) with a sub-area expanded around a distinct semi-circular carbon-dense structure that is reovirus-positive.

(C and D) In (C) is a surface rendering of a partial stack of the corresponding density looking down the Z axis and in (D), the same volume as (C), tilted to show the 3D structure.

All images were generated with Chimera (Pettersen et al., 2004).