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. 2020 Jul 29;40(7):BSR20200986. doi: 10.1042/BSR20200986

Figure 11. Pinocembrin blocked LPS-induced TLR4/MD2 association.

Figure 11

(A and B) Co-immunoprecipitation assay was carried out to investigate the effect of pinocembrin on the formation of TLR4/MD2 complex in RAW264.7 cells. RAW264.7 cells were pretreated with pinocembrin (0–150 μM) for 2 h and then stimulated with LPS (1 μg/ml) for 22 h. Ultimately, cell lysates were immunocoprecipitated with anti-TLR4 antibody and then subjected to immunoblotting analysis of MD2 and TLR4. The immunocoprecipitative immunoblots were analyzed by densitometric analysis of the MD2/TLR4 ratio. Data were expressed as the mean ± SD (n=3). *P<0.05, ***P<0.001 vs. LPS group; ###P<0.001 vs. Vehicle group.