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. 2020 Jul 29;40(7):BSR20200986. doi: 10.1042/BSR20200986

Figure 8. Pinocembrin inhibited the expression of pro-inflammatory mediators in vitro.

Figure 8

(A) Effect of pinocembrin on cell viability and the secretion of NO in LPS-induced RAW264.7. Cells were treated with pinocembrin (0–200 μM) for 24 h, and then cell viability was measured. Also, the production of NO was determined. (B) RAW264.7 cells were treated as described in ‘Materials and Methods’ section. Protein expression levels of iNOS, COX-2 and TNF-α were determined by immunoblotting analysis. Expression was normalized to β-actin. (C) Effect of pinocembrin on the expression of pro-inflammatory mediators mRNA in LPS-induced RAW264.7 cells. All the expressions were normalized to β-actin. Data were exhibited as the mean ± SD (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. LPS group; ###P<0.001 vs. Vehicle group.