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. 2020 Jul 29;40(7):BSR20200986. doi: 10.1042/BSR20200986

Figure 9. Pinocembrin inhibited TLR4/NF-κB signaling in vitro.

Figure 9

RAW264.7 cells were exposed to pinocembrin (150 μM) for 2 h and then treated with LPS (1 μg/ml) for an additional 22 h. (A) Effect of pinocembrin on TLR4 pathway-related proteins expression in LPS-treated RAW264.7 cells was determined by immunoblotting analysis. (B) RAW264.7 cells were transfected with pGL4.32[luc2P/NF-κB-RE/Hygro] luciferase reporter vector and cell extracts were assayed for luciferase activity as described in the ‘Materials and Methods’ section. (C) Immunofluorescence staining was conducted to detect the NF-κB p-p65 nuclear translocation. Scale bar corresponds to 25 or 5 μm. Data were exhibited as the mean ± SD (n=3). **P<0.01, ***P<0.001 vs. LPS group; ###P<0.001 vs. Vehicle group.