Table 2. Fasting metabolic parameters and organ weights in ovary-intact (INT) and ovariectomized (OVX) WT, αKO and βKO mice.
Two (i.e., INT vs OVX) by three (i.e., WT vs αKO vs βKO) ANOVA were performed to determine main effects and interaction effects, which were followed by post hoc Tukey’s tests where appropriate.
| WT-INT | WT-OVX | αKO-INT | αKO-OVX | βKO-INT | βKO-OVX | |
|---|---|---|---|---|---|---|
| Insulin (ng/mL) | 0.73 ± 0.21 | 0.79 ± 0.17 | 1.29 ± 0.25 | 0.77 ± 0.18 | 0.68 ± 0.08# | 1.27 ± 0.27#* |
| Glucose (mg/dL) | 155.7 ± 38.3 | 212.7 ± 29.9 | 219.3 ± 31.8 | 242.9 ± 16.8 | 208.5 ± 14.2 | 213.7 ± 15.8 |
| Cholesterol (mg/dL) | 56.3 ± 7.9 | 62.1 ± 6.4 | 68.7 ± 10.8 | 80.0 ± 6.1 | 74.3 ± 3.5 | 86.4 ± 5.6*$ |
| LDL (mg/dL) | 4.83 ± 1.08 | 5.57 ± 0.90 | 5.14 ± 0.59 | 7.14 ± 1.06 | 4.80 ± 0.20 | 8.80 ± 0.79*$ |
| HDL (mg/dL) | 25.2 ± 2.5 | 32.9 ± 2.8 | 32.1 ± 5.4 | 41.3 ± 2.2 | 34.9 ± 1.3# | 39.4 ± 2.0* |
| Triglycerides (mg/dL) | 133.5 ± 5.4 | 153.6 ± 9.9 | 133.57 ± 10.0 | 149.3 ± 5.9 | 140.0 ± 5.5 | 148.6 ± 9.3 |
| NEFA (mmol/L) | 0.85 ± 0.15 | 0.88 ± 0.13 | 0.70 ± 0.12 | 0.77 ± 0.07 | 0.74 ± 0.07 | 0.88 ± 0.07 |
| Estradiol (pg/mL) | 13.91 ± 1.76 | 14.22 ± 1.44 | 34.8 ± 11.7#$ | 9.97 ± 2.45^ | 10.0 ± 1.8 | 9.17 ± 2.38 |
| Uterine weight (mg) | 9.17 ± 1.08 | 2.23 ± 0.18* | 8.00 ± 1.62 | 2.44 ± 0.65* | 10.10 ± 1.14 | 4.79 ± 1.24* |
| Liver weight (g) | 1.14 ± 0.12 | 1.12 ± 0.07 | 1.36 ± 0.14 | 1.17 ± 0.13 | 1.27 ± 0.08 | 1.32 ± 0.07 |
| Liver TG (mgTAG/g) | 47.19 ± 7.30 | 36.23 ± 4.99 | 42.40 ± 8.80 | 44.40 ± 10.70 | 77.70 ± 4.43# | 61.08 ± 9.01$ |
*
p<0.05;
^
p<0.06 different from INT within genotype,
#
p<0.05 compared to αKO within INT or OVX state;
$
p<0.05 different from WT within INT or OVX state. Values are expressed as mean ± SEM.