Table 1.

Comparison of tissue biopsy and liquid biopsy in sarcoma

	Advantages	Limitations
Tissue biopsy	1. The gold standard of diagnosis, classification and staging of sarcoma 12, 13 2. Guides treatment in sarcoma 12, 13 3. Well-developed technical process and widely used in the clinic 12, 13	1. Invasive procedure 14, 15 2. Difficult to detect intratumoral and intertumoral heterogeneity with a single sample from a single site 16, 17 3. Possible clinical complications associated with tissue biopsy 14, 15 4. Difficult to sample certain parts of the body 12, 13 5. Difficult to sample continuously during the follow-up period 12, 13
Liquid biopsy	1. Non-invasive procedure 19, 22 2. Easily repeated sampling during the follow-up period 19, 22 3. Body fluid samples can reflect a panoramic view of the tumor in real time, including the primary and metastatic sites 19, 22 4. Dynamic evaluation of therapeutic effects 19, 22 5. Formation of personalized therapy based on tumor heterogeneity 19, 22 6. Easy sampling and not limited by the anatomic location of the tumor 19, 22	1. Difficult to find sensitive and specific biomarkers due to the high heterogeneity of sarcoma 13, 16, 195 2. Significance of biomarkers and clinicopathological features in sarcomas have not been fully established 16, 195 3. Differences between samples and sampling processes lead to poor consistency in results between different studies 16, 195