Table 2.
Primer/Oligonucleotide | Sequence (5'-3') |
---|---|
miR-154-5p F | GCGCGCGTAGGTTATCCGTGTTG |
miR-154-5p R | ATCCAGTGCAGGGTCCGAGG |
miR-154-5p RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGAAGG |
H-U6 F | CTCGCTTCGGCAGCACA |
H-U6 R | AACGCTTCACGAATTTGCGT |
H-U6 RT | AACGCTTCACGAATTTGCGT |
miR-154-5p mimic sense | UAGGUUAUCCGUGUUGCCUUCG |
miR-154-5p mimic anti-sense | AAGGCAACACGGAUAACCUAUU |
miR-154-5p mimic NC sense | UUCUCCGAACGUGUCACGUTT |
miR-154-5p mimic NC anti-sense | ACGUGACACGUUCGGAGAATT |
miR-154-5p inhibitor sense | CGAAGGCAACACGGAUAACCUA |
miR-154-5p inhibitor NC sense | CAGUACUUUUGUGUAGUACAA |
CUL2 siRNA sense | CCUCUUACUCAGGCUCCUUTT |
CUL2 siRNA anti-sense | AAGGAGCCUGAGUAAGAGGTT |
CUL2 siRNA NC sense | UUCUCCGAACGUGUCACGUTT |
CUL2 siRNA NC anti-sense | ACGUGACACGUUCGGAGAATT |