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. 2020 Jul 9;11(18):5329–5344. doi: 10.7150/jca.45394

Figure 5.

Figure 5

miR-941 is Highly Expressed in LSCC Cell Lines and Promotes Proliferation and Invasion. (A) Cellular and exosomal RNA was extracted from two LSCC cell lines (FD-LSC-1 and Tu 686) and a normal cell line (HOK), and miR-941 levels were quantified via qRT-PCR. Data were normalized to levels of U6 and compared with the nontumor cell line HOK. (B) MiR-941 expression was detected in FD-LSC-1 and Tu 686 by qRT-PCR after transfection of miR-941 mimics, miR-941 inhibitor, or associated controls. (C) A CCK8 assay was performed to determine the effect of miR-941 gain or loss on cell proliferation. (D) The effect of miR-941 gain or loss on cell invasion was evaluated by using a Transwell invasion assay. Representative images are shown. Cell numbers were counted by randomly selecting five fields at 100× magnification. Data are presented as a mean ± SD. ***P < 0.001, **P < 0.01, *P < 0.05.