TABLE 1.

Station depth, category, and number of reads obtained per sample after sequencing, removing primers, pair‐assembling, quality filtering, primer mapping, and chimera removal for the teleo region

Sample	Station depth (m)	Category	Raw	Retained after primer checking	Retained after merging	Retained after quality filtering	Retained after mapping to teleo region	Retained after chimera removal	% of retained reads for analysis
Sample_01	27	Shallow	127,549	100,839	99,036	99,036	95,240	95,240	74.67
Sample_02	1,315	Deep	99,724	96,995	90,080	90,080	89,206	89,206	89.45
Sample_03	764	Deep	67,867	49,789	39,229	39,229	28,896	28,896	42.58
Sample_04	46	Shallow	93,699	89,918	85,894	85,894	83,153	83,153	88.74
Sample_05	43	Shallow	157,845	150,348	145,987	145,987	100,388	100,387	63.60
Sample_06	180	Deep	120,961	116,014	103,982	103,982	101,419	101,418	83.84
Sample_07	508	Deep	55,396	36,121	33,605	33,605	16,062	16,061	28.99
Sample_08	1,373	Deep	104,158	77,717	71,716	71,716	65,091	65,091	62.49
Sample_13	91	Medium	138,472	134,943	122,420	122,420	117,565	117,562	84.90
Sample_14	735	Deep	66,247	50,282	21,624	21,624	18,813	18,813	28.40
Sample_15	639	Deep	98,224	96,676	91,079	91,079	89,581	89,581	91.20
Sample_16	25	Shallow	94,195	92,482	92,074	92,074	87,100	87,100	92.47
Sample_17	741	Deep	60,308	38,492	24,125	24,125	20,355	20,355	33.75
Sample_18	127	Medium	101,688	99,550	98,456	98,456	97,918	97,918	96.29
Sample_19	38	Shallow	119,881	113,505	104,320	104,320	99,181	99,181	82.73
Sample_20	1,285	Deep	111,757	107,998	103,660	103,660	96,993	96,993	86.79
Sample_21	300	Deep	134,490	132,496	130,290	130,290	121,976	121,976	90.70
Sample_22	33	Shallow	88,044	78,156	50,143	50,143	43,798	43,798	49.75
Sample_23	968	Deep	52,240	39,687	16,584	16,584	12,090	12,090	23.14
Sample_24	169	Deep	104,423	97,858	77,320	77,320	65,788	65,788	63.00
Sample_25	23	Shallow	89,199	79,999	68,124	68,124	59,414	59,414	66.61
Sample_26	132	Deep	110,206	106,817	106,547	106,547	105,436	105,436	95.67
Sample_27	1,003	Deep	99,856	94,244	75,172	75,172	69,577	69,577	69.68
Sample_28	112	Medium	100,002	98,722	97,656	97,656	97,462	97,462	97.46
Sample_29	38	Shallow	87,452	73,824	61,161	61,161	51,590	51,590	58.99
Sample_30	24	Shallow	155,459	153,556	153,517	153,517	152,877	152,877	98.34
Sample_31	100	Medium	91,723	77,592	65,748	65,748	57,283	57,283	62.45
Sample_32	185	Deep	76,396	64,566	52,863	52,863	35,175	35,175	46.04
Sample_33	480	Deep	81,771	74,171	59,470	59,470	54,788	54,788	67.00
Sample_34	104	Medium	70,037	56,967	54,193	54,193	45,712	45,712	65.27
Sample_35	28	Shallow	124,438	122,670	122,076	122,076	113,812	113,812	91.46
Sample_36	25	Shallow	108,071	107,183	107,166	107,166	106,898	106,898	98.91
Sample_37	96	Medium	138,090	134,214	132,943	132,943	131,644	131,644	95.33
Sample_38	590	Deep	89,791	77,675	72,468	72,468	59,562	59,562	66.33
Sample_39	1,010	Deep	38,021	26,356	21,918	21,918	16,839	16,839	44.29
Sample_40	108	Medium	79,924	74,147	67,941	67,941	64,152	64,152	80.27
Sample_41	26	Shallow	119,488	117,958	117,881	117,881	116,414	116,414	97.43
Sample_42	30	Shallow	135,958	133,840	46,726	46,726	46,640	46,640	34.30
Sample_43	104	Medium	115,584	109,058	76,209	76,209	71,558	71,558	61.91
Sample_44	185	Deep	25,519	24,976	8,064	8,064	7,755	7,755	30.39
Sample_45	33	Shallow	115,109	113,582	99,454	99,454	93,219	93,219	80.98
Sample_46	90	Medium	121,100	119,555	119,036	119,036	106,640	106,636	88.06
Sample_47	675	Deep	68,697	55,896	19,019	19,019	6,716	6,716	9.78
Sample_48	110	Medium	94,499	91,697	90,715	90,715	89,305	89,305	94.50
Sample_01NOBP	27	Shallow	115,003	97,454	93,987	93,987	82,749	82,748	71.95
Sample_27NOBP	1,003	Deep	88,793	78,114	51,755	51,755	43,902	43,902	49.44
Sample_32NOBP	185	Deep	57,276	38,142	29,015	29,015	26,686	26,686	46.59
Sample_47NOBP	675	Deep	46,283	35,228	7,304	7,304	1857	1857	4.01
TOTAL			4,333,558	3,989,131	3,497,691	3,497,691	3,211,081	3,211,071
AVERAGE_all			98,489.95	90,662.07	79,492.98	79,492.98	72,979.11	72,978.89	69.52