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Initial velocities (V0) [nM/min] of R21-Mod duplex formation calculated for the hDicer-assisted annealing reactions
| R21 | miRNA-like duplex | siRNA-like duplex | |
|---|---|---|---|
| Mod18 | 0.74 ± 0.06 | 0.37 ± 0.08 | < 0.01 |
| Mod23 | 0.35 ± 0.07 | 0.27 ± 0.11 | 0 |
| Mod33 | 0.05 ± 0.01 | < 0.01 | 0 |
