Fig. 5.
Types of vesicle–plasmalemma interactions measured by high-resolution patch-clamp membrane capacitance measurements in cultured rat astrocytes. a Representative examples of upward and downward steps in the imaginary (Im) and real (Re) component of the admittance signal represent elementary events of exo- and endocytosis: (i) full exocytosis (ii) full endocytosis (iii) reversible exocytosis (iv) reversible endocytosis; (v) an event of reversible exocytosis that exhibited projection to the Re trace, indicating the formation of a highly resistive narrow fusion pore enabling measurement of fusion pore conductance (Gp). Asterisks denote calibration pulses. For details, see “Materials and methods”. b and c Individual events of reversible exocytosis in control (b) and IFNγ-treated astrocytes (c) marked by the arrows in Im component of the admittance trace signal