Fig. 7.

Astrocyte treatment with IFNγ alters fusion pore geometry and pore kinetics in reversible exocytosis. a Fusion pore conductance (G_p) and diameter (2r) of reversible exo- (Rev Exo) and endocytotic vesicles (Rev Endo) establishing a narrow fusion pore at rest and after ATP stimulation of controls and IFNγ-treated astrocytes. b Fusion pore dwell time (dwell t) measured in reversible exo- and endocytotic vesicles at rest and after ATP stimulation in controls and IFNγ-treated astrocytes. Numbers at the bottom of the bars denote the number of vesicles (a), and exo- and endocytotic events examined (b). Note an increase in fusion pore diameter and open pore dwell time in vesicles undergoing transient exocytosis at rest and after ATP stimulation in IFNγ-treated astrocytes. *P < 0.05, **P < 0.01, ***P < 0.001 (Mann–Whitney U test). c Plots depicting the relative frequency distribution of fusion pore conductance (G_p) and pore diameter (pore 2r) in reversible exocytotic vesicles establishing a narrow fusion pore at rest and after ATP stimulation in controls and IFNγ-treated astrocytes