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. 2020 Feb 23;28(4):465–474. doi: 10.1177/1708538120904297

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Calcified A7r5 cells treated with E2 and its antagonist: (a) calcified A7r5 cells treated with E2 and its antagonist, with ethanol as a vehicle control (eth group), alizarin red staining showed that calcification reduction induced by E2 were alleviated by ICI182780 treatment (×100); (b and c) real-time PCR and western blotting demonstrated that HIF-1α were upregulated in the A7r5 calcification treatment, and this augment of HIF-1α in calcification was inhibited by E2. (n = 3) *p < 0.05, **p < 0.01.

Abbreviations: cal: calcification; eth: ethanol vehicle.