Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 29;8:120. doi: 10.1186/s40478-020-00997-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — The differentiation of BCAS1(+) cells was affected by p-α-syn-immunoreactive inclusions in the frontal cortices of MSA brains. a Serial sections of brain tissues from the frontal cortices of an aged non-neurodegenerative disease control (upper) and an MSA patient (lower) which were immunostained with antibodies against BCAS1, MBP, p-α-syn, and NeuN. Scale bar = 100 μm. The regions marked by dotted squares are magnified in the right upper corners. The magnified images of BCAS1 staining show altered morphology of BCAS1(+) cells in the MSA case. Images of control and MSA cases are provided from case 21 and case 2 (Additional file 1: Table S1), respectively. b, e Quantification of BCAS1(+) cell counts (b), MBP(+) cell counts (c), p-α-syn(+) area (d), and NeuN(+) cell counts (e) in control and MSA brains. Mean ± SEM; control, N = 6, MSA, N = 9; Mann–Whitney, p** < 0.01, p*** < 0.001. f Illustration of oligodendroglial differentiation showing the morphological classification of early-stage and late-stage BCAS1(+) cells. g Immunostaining of an aged non-neurodegenerative disease control (case 21, Additional file 1: Table S1) with the anti-BCAS1 antibody showing representative images of early-stage (a, b) and late-stage (c, d) BCAS1(+) cells in the frontal cortex. Scale bar = 500 μm (low-power field, left) and 20 μm (high-power field, right). (H) Immunostaining of the serial sections from the frontal cortices of two different MSA cases (cases 1 and 2, Additional file 1: Table S1) showing the morphological variety of BCAS1(+) cells and their association with the density of the p-α-syn(+) area. Black arrowheads indicate late-stage BCAS1(+) cells, whereas white arrowheads indicate early-stage BCAS1(+) cells. Case 1 with sparse cortical p-α-syn pathology shows multi-processed mature BCAS1(+) cells. In contrast, case 2 with notable p-α-syn pathology shows round BCAS1(+) cells with few processes. Scale bar = 100 μm (low-power field, left and middle) and 20 μm (high-power field, right). (I) Linear regression analysis showing a negative correlation between the number of late-stage BCAS1(+) cells and the density of p-α-syn(+) in the frontal cortices of MSA patients (N = 9, Spearman r = − 0.8000, p = 0.0138)