Fig. 5.

Validation of the circRNA-sequencing results. a Expression levels of hsa_ circ_0000419, hsa_circ_0001523, hsa_circ_0000825, and chrM: 14131–15,754- in the irradiation group (group B) with that in the non-irradiation group (group A). The results of quantitative real-time PCR (qRT-PCR) were evaluated by 2^-△△CT method. Results were represented as means ± standard deviation (SD). *p-value < 0.05. b CircRNAs were resistant to RNase R digestion, whereas the linear RNAs were sensitive to RNase R digestion. Four circRNAs were examined, which were qualified in expression level. And FBXW7, GAPDH, β-actin, and 18S rRNA were used as negative controls and known circRNA FBXW7 was used as a positive control. The results of quantitative Real-time PCR (qRT-PCR) were evaluated by 2^-△CT method. Results were represented as means ± standard deviation (SD) *p-value < 0.05, ** p-value < 0.001