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. 2020 Jul 29;10:12737. doi: 10.1038/s41598-020-69673-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron microscopic (TEM) analysis of mitochondria in brain tissues. (A) Diagrammatic representation of dissection of adult rat brain and tissue sectioning for TEM. (B) representative TEM images of sham, vehicle and sovateltide (Sova) treated rat right hemisphere (RH) brain tissues at 24 h and day 7 post MCAO (supporting images are provided in Fig. S4). Black arrows indicate representative mitochondria. Image magnification 3,000×, scale bar = 0.2 µm. (B) Measurement of mitochondrial cross-sectional area × number at 24 h and day 7 post MCAO, respectively. (C) Measurement of percent mitochondrial to tissue area ratio at 24 h and day 7 post MCAO, respectively. Values are expressed as mean ± SEM (B,C).