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. 2020 Jul 29;10:12737. doi: 10.1038/s41598-020-69673-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In situ PCR analysis of mitochondrial biogenesis in brain tissues. (A) Diagrammatic representation of dissection of adult rat brain and tissue sectioning for in situ PCR. (B) representative in situ PCR images of sham, vehicle and sovateltide (Sova) treated rat right hemisphere brain tissues at day 7 post MCAO (supporting images are provided in Fig S5). Red fluorescence indicates amplified MT-ATP8 DNA in mitochondria. Image magnification 200×. (B) fluorescence intensity graph of MT-ATP8 DNA. Values are expressed as mean ± SEM.