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. 2020 Jul 29;10:12663. doi: 10.1038/s41598-020-69401-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the metabolic processes relevant to the mxaF fae transposon mutagenesis study. XoxF1 and XoxF2 oxidize methanol to formaldehyde, which accumulates to lethal levels in the fae mutant strain. If a process required for XoxF-dependent methanol oxidation is disrupted by a transposon insertion, formaldehyde is reduced or eliminated, and cells use succinate for growth. A dashed line around the formaldehyde transporter is to indicate that this function has not been demonstrated.