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. 2020 Jul 29;10:12663. doi: 10.1038/s41598-020-69401-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth of mutant strains lacking genes identified through transposon mutagenesis in medium containing methanol and La³⁺. Growth of M. extorquens AM1 wild-type is represented by blue circles and depicted mutant strains (red triangles) have the following genes deleted: (a) MexAM1_META1p1771, mxaD homolog; (b) MexAM1_META1p1746, orf6; (c) MexAM1_META1p1747, orf7; (d) MexAM1_META1p2024, homospermidine synthase; (e) MexAM1_META1p2359, ABC transporter of unknown function; (f) MexAM1_META1p3908, aminopeptidase. Representative data from biological triplicates are shown. One-way analysis of variance (ANOVA) determined that growth rate differences between mutant and parent strains are significantly different (p < 0.005).