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. 2020 Jul 29;10:12663. doi: 10.1038/s41598-020-69401-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the lut cluster. (a) Genomic map of the lut genes (MexAM1_META1p1778 to MexAM1_META1p1787). Black, genes encoding the ABC transport system; green, gene encoding the TonB-dependent transporter; blue, genes encoding putative periplasmic proteins identified by transposon mutagenesis; white, lanmodulin and additional genes encoding periplasmic proteins not identified by transposon mutagenesis. (b) Model for Ln transport. The three-dimensional structures of monomers of LutH and LutA were predicted using homology modeling (HHpredserver and MODELLER)³⁷ and homodimers of the ABC transporter were predicted using GalaxyHomomer³⁸. (c) Growth of mxaF and mxaF lut mutant strains with 2 μM LaCl₃. Graphs depict representative data from three biological replicates. Growth rate averages and standard deviations are shown in Table 2.