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. 2020 Jul 29;10:12663. doi: 10.1038/s41598-020-69401-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth studies and measurement of La³⁺ uptake suggest La³⁺ storage capability. (a) Growth of wild type (red dashed circles), lutH (white triangles), lutA (blue squares), lutE (gray diamonds), and lutF (black circles) strains grown with limiting succinate (3.75 mM), methanol (125 mM), and 2 μM LaCl₃. Data are the average of three biological replicates. Standard deviations between biological replicates were less than ± 0.02. (b) La³⁺ concentrations (μM) in culture supernatants of lut mutant strains depicted in same color code as in (a). Data depict the average of three biological replicates with error bars showing the standard deviation. One-way analysis of variance (ANOVA) followed by a t-test was used to represent statistical significance. *The p-value is < 0.005.