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. 2020 Jul 29;10:12663. doi: 10.1038/s41598-020-69401-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Visualization of La³⁺ storage. TEM of ultrathin sections of wild-type cells harvested at OD₆₀₀ of ~ 0.6 after growth with 3.75 mM succinate and 125 mM methanol containing (a, b) and lacking (e, f) 20 µM La³⁺. (c, g) Elemental analysis of electron dense deposits from cells grown with (c) and without (g) La³⁺. (d) High-resolution transmission electron microscopy analysis of the wild-type strain shows an atomic lattice structure in electron-dense areas suggesting that La³⁺ is embedded in cytoplasmic crystals.