Table 2.
Growth parameters for strains grown in methanol medium with La3+.
| Strain | Growth rate (h−1)a in MeOH + La3+ |
|---|---|
| Growth of control strains | |
| Wild type | 0.16 ± 0.01 |
| mxaF | 0.16 ± 0.01 |
| xoxF1 | 6–9 h lag, 0.07 ± 0.00 |
| xoxF1 xoxF2 | 6 h lag, 0.04 ± 0.01 |
| mxaF xoxF1 xoxF2 | 6 h lag, 0.04 ± 0.00 |
| Growth of strains lacking novel genes identified in genetic selection | |
| MexAM1_META1p0863 | 0.14 ± 0.00 |
| MexAM1_META1p1771 | 0.11 ± 0.01 |
| orf6 | 0.03 ± 0.00 |
| orf7 | 9 h lag, 0.08 ± 0.01 |
| MexAM1_META1p2359 | No growth |
| hss | 6 h lag, 0.10 ± 0.01 |
| MexAM1_META1p3908 | 15 h lag, 0.07 ± 0.00 |
| Growth of strains lacking genes with known function | |
| pqqBCDE | No growth |
| pqqF | No growth |
| cycK | No growth |
| ccmB | No growth |
| ccmC | No growth |
| Growth of strains lacking lanthanide utilization and transport genes | |
| lutA | 0.02 ± 0.01, 96 h; 0.08 ± 0.01 |
| mxaF lutA | 0.02 ± 0.00 |
| lutB | 0.02 ± 0.00, 87 h; 0.05 ± 0.01 |
| mxaF lutB | 0.02 ± 0.00 |
| lutC | 9 h lag, 0.11 ± 0.01 |
| mxaF lutC | 9 h lag, 0.11 ± 0.01 |
| lutE | No growth |
| mxaF lutE | No growth |
| lutF | No growth |
| mxaF lutF | No growth |
| lutG | 12 h lag, 0.03 ± 0.00 |
| mxaF lutG | 12 h lag, 0.03 ± 0.00 |
| lutH | 0.16 ± 0.00 |
| mxaF lutH | No growth |
aData for a minimum of three biological replicates are reported.
±Indicates standard deviations.