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. 2020 Jul 29;10:12778. doi: 10.1038/s41598-020-69675-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of PBI-4547 intervention on NAFLD development. (A) Representative image of HE-stained liver section of STD, HFD and HFD + PBI-4547 mice. (B) Scoring of steatosis, ballooning, inflammation and total NAFLD score in liver sections of the mice. (C) Quantification of hepatic triglyceride and cholesterol levels. (D) Relative expression levels of genes involved in fibrosis, inflammation and with adipokine. Mean expression of qRT-PCR data was set to 1 for HFD group. Data are presented as mean ± SEM (n ≥ 6 per group, one-way ANOVA with Dunnett’s multicomparison test vs HFD group).