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. 2020 Jul 29;11:3784. doi: 10.1038/s41467-020-17652-0

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

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Fig. 7 — Phage DMS3m or JBD8 spot titration from left to right (tenfold serial dilutions) on lawns of P. aeruginosa strains expressing the indicated CRISPR-Cas system (X-axis, type I-C, I-F, or I-E), with a crRNA targeting the indicated phage and the indicated Acr or empty vector (Y-axis). During screening of all candidate Acr proteins against all three systems, each combination was screened once. Upon detecting inhibition, positive results were visually confirmed in triplicate.