Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 29;10:12685. doi: 10.1038/s41598-020-69698-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The protein phosphatase activity of PTEN confers an epithelial phenotype. (A) Live phase contrast micrographs show BT549 cells stably transfected with wild-type PTEN, PTEN G129E, C124S, or the vector alone. (B) Stably transfected BT549 cells were subjected to immunoblot analysis. Actin served as a loading control. Splice V3: splice variant 3. (C) BT549 cells were cultured in suspension for 7 days and mammosphere formation was counted. Transfection with wild-type PTEN and G129E but not C124S reduced mammosphere formation. N = 24, *P < 0.01. (D) BT549 cells treated with the PI3K inhibitor wortmannin (1 µM), the Akt 1/2 inhibitor (10 µM), or vehicle alone for 48 h were analyzed by immunoblotting for Akt phosphorylation as well as the mesenchymal and epithelial markers. Actin served as a loading control. (E) Live phase contrast micrographs show control and inhibitor-treated cells.