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. 2020 Jul 29;10:12685. doi: 10.1038/s41598-020-69698-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Depletion of Abi1 in breast cancer cells suppresses the EMT and cancer stem cell activity. (A) BT549 cells were stably transfected with shRNAs targeting human Abi1 (Abi1 knockdown, KD) or the scrambled control (SC). Live phase contrast micrographs of Abi1 knockdown and scrambled control BT549 cells. (B) The cells were analyzed by immunoblotting with actin served as a loading control. (C) Control and Abi1 knockdown BT549 cells were cultured in suspension for 7 days and mammosphere formation was counted. Knockdown of Abi1 reduced mammosphere formation. N = 22, *P < 0.01. (D) Giemsa-staining showed BT549 cells migrated to the bottom of the transwell filter in Matrigel invasion assay. (E) The cells migrated to the bottom of the filter were counted and plotted. N = 24, *P < 0.001.