Table 4.
In vitro antibacterial synergistic activity of compound 40 on recombinant isogenic E. coli strains evaluated by the disk diffusion method (See Materials and Methods for details).
| Plasmid carried by the E. coli DH5α strain | Diameter of growth inhibition zone (mm) | ||||
|---|---|---|---|---|---|
| Ampicillin (10 µg) | Ampicillin (10 µg) + 40 (80 µg) | Ampicillin (10 µg) + avibactam (4 µg) | Ampicillin (10 µg) + EDTA (70 µg) | 40 (80 µg) | |
| pLBII-CTX-M-15 | ≤ 6 | 16 | 26 | – | ≤ 6 |
| pLBII-KPC-2 | 9 | 11 | 22 | – | ≤ 6 |
| pLBII-AmpCP. aeruginosa | 19 | 19 | 25 | – | ≤ 6 |
| pLBII-VIM-2 | 9 | 17 | – | 24 | ≤ 6 |
| pLBII-NDM-1 | 16 | 18 | – | 21 | ≤ 6 |
DH5α strain was transformed with a derivative of the pLB-II vector, in which the β-lactamase gene was cloned)44. Avibactam and EDTA were used as inhibition controls for SBLs and MBLs, respectively.
– not determined.