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. 2020 Jul 29;10:12713. doi: 10.1038/s41598-020-69620-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern of N starvation-induced genes screened from UTEX395 based on RNA-Seq data from ArM0029B. (A) Chlorella UTEX 395 grown in N-rich media was transferred into N-deficient media and then cultivated for 3 days. Total RNA was extracted from the cells harvested every day after the transfer, and RT-PCR was performed using scaffold-specific primers. (D0) before transfer; (D1) 1 day after transfer; (D2) 2 days after transfer; (D3) 3 days after transfer. Both CvUbi and CvAct1 were used for normalization.