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. 2020 Jul 29;10:12713. doi: 10.1038/s41598-020-69620-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Construction of hG-CSF expression vectors controlled by CvNDI promoters. Using pJKS136 as a backbone, the RAmy3D promoter (PRAmy3D) was replaced with either CvNDI1 (pSK401 and pSK402) or CvNDI2 (pSK403 and pSK404). The hG-CSF sequence of pJKS136 (white box) was replaced with a codon-optimized hG-CSF sequence (yellow box) for Chlorella. The signal peptide of a putative cellulase (SP_c), screened from our secretome data from UTEX395, was introduced into pSK401 and pSK403 for the transformation of UTEX 395. The signal peptide of a Ras-related RABF1 (SP_R), screened from our secretome data of ArM0029B, was introduced into pSK402 and pSK404 for the transformation of ArM0029B. The black bar indicates a length of 100 bp.