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. 2020 Jul 29;10:12713. doi: 10.1038/s41598-020-69620-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular characterization of transgenic Chlorella spp. (a) Genomic DNA PCR of the transgenic Chlorella, UTEX 395 (left panel) and ArM0029B (right panel), using hG-CSF primers. NC, negative control; PC, positive control. Vertical black lines indicate the grouping of gels cropped from different parts of the same gel. (b) Expression of an hG-CSF transcript by N deficiency in the transgenic UTEX 395. (c) Expression of hG-CSF transcript by N deficiency in the transgenic ArM0029B. Transgenic Chlorella grown in standard media was transferred to N-deficient media and harvested at 0 days (D0), 2 days (D2), and 3 days (D3) after the transfer. CvUbi gene was used for normalization.