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. 2020 Jul 29;10:12728. doi: 10.1038/s41598-020-69026-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Multicellular Cancer Tumor Spheroids of breast cancer cell line MCF7 diameter along time. We performed three independent biological replicates of the MCF7-MCTS culture kinetic denoted as N1, N2 and N3. Points and bars represent the average Feret diameter with their respective standard deviation for each replicate and time-point. Values can be found in Supplementary Table S1. (b) Immunophenotyping with flow cytometry analysis measuring MKI67 and p27 biomarkers at days 6 and 19, expression of both markers for the two-time points showed statistical difference with a p value < 0.05 using a non-paired t-student test with equality of variances.