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. Author manuscript; available in PMC: 2020 Nov 15.
Published in final edited form as: Science. 2020 May 15;368(6492):eaay5051. doi: 10.1126/science.aay5051

Fig 1. Computational design of RSV epitope-focused immunogens.

Fig 1

(A) Prefusion RSVF structure (PDB 4JHW) with sites 0, II and IV highlighted. (B) Computational protein design strategies. Approach 1: Design templates were identified in the PDB, followed by in silico folding and design. Approach 2: A template-free design approach was developed (“TopoBuilder”) to tailor the protein topology to the motif’s structural constraints. Bottom: Computational models of designed immunogens. (C) Cocktail formulations of designed immunogens displayed on nanoparticles elicit nAbs focused on three non-overlapping epitopes. SSEs: secondary structure elements; α: alpha-helix; β: beta strand; ddG: computed binding energy.