FIGURE 4.

Expression analysis of the inducible genes by real time quantitative PCR (RT‐qPCR) in the site‐specific integration (SSI) lines relative to T5 negative control. (a–c) AtDREB1a expression analysis at room temperature (white bars) or upon cold‐induction (20 hr in ice pack, magenta bars) in T0, T1, and T2 plants. (d–f) pporRFP expression at room temperature (white bars) or upon heat‐induction (42°C for 3 hr; red bars) in T0, T1, and T2 plants. The SSI line numbers are given on x‐axis. The values are the average of two biological replicates with standard error indicated as the error bars